His brothers: François, Charles. He married Marie de Clèves with whom he had a daughter: Catherine. 
From his second marriage with Charlotte de La Trémoille: a daughter Eléonore and a son: Henri II. 

His father's death at Jarnac left him and his cousin Henry of Navarre (the future Henry IV) as leaders of the Huguenots. After the Peace of Saint-Germain (1570) Henri retired to Béarn and married Marie de Clèves. She died after giving birth to his daughter Catherine (1574-93). Caught in Paris during the Massacre of St Bartholomew's Day (1572), he had been forced to profess Catholicism. Nominally governor of Picardy, he was kept under surveillance until, in 1574, he escaped to Alsace and began raising troops for the Huguenots. Invading France with a horde of mercenaries to collaborate with the Duc d'Alençon, he was disappointed at the terms which Alençon made with the government (1576). In the next civil wars he became rather an embarrassment to Henry of Navarre, set himself up as chief of the most fanatical Huguenots, and failed conspicuously in his travels abroad in search of foreign help (1580) and in his campaign of 1585 in western France when he was driven to take refuge in Guernsey. Returning to France, he married, in 1586, Charlotte de La Trémoille (1565-1629), who renounced Catholicism for him and bore him a daughter, Éléonore (1587-1619) and an heir Henri II in 1588 after his death. He had been wounded at the Battle of Coutras in October 1587, but it was suspected that Charlotte had poisoned him. It was in prison that she gave birth to his only son: Henri II.